General information:
Substance group: Tricyclic antidepressant ATC: N06AA09
Trade names: Amitriptylin*®, Saroten®, Syneudon®, Tryptizol®
available dosages: film tablets 10 / 25 / 50 / 75 / 100 mg, hard capsules retarded 25 / 50 / 75 mg,
Take-in solution 40 mg/ml, solution for injection 25 mg/mL
Reference ranges :
Amitriptyline: 50 – 200 µg/L
Nortriptyline: 20 – 200 µg/L
Amitriptylin + Nortriptyline: 80 – 200 µg/L
Risky plasma levels: from 300 – 400 µg/L increases the risk of disturbances of the cardiac excitation line
Toxic plasma concentrations: Amitriptyline: 500 µg/L – 750 µg/L
Nortriptyline: 300 µg/L
Pharmacokinetics:
T1⁄2 : 10-28 h
Tmax : 2–6 h
Bioavailability: 50%
Proteinbinding: 94–97%
Clearance: 800 mL/min
Excretion: urine: > 95%
Metabolism: hepatic
Distribution volume: 14–18 L/kg KG
Time to steady state: 3–8 days
Sampling:
Material: Serum (Monovette brown) Minimum volume for analysis: 500µl
Recommendation: trough level (directly before the next intake)
Requirement (internal): about Orbis requirement (external):
Analytics:
Test principle: liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS)
Protein precipitation with acetonitrile, chromatographic separation, mass spectrometry
Analysis according to mass charge ratio
Analysis system: triple stage Quadrupol mass spectrometer (LC-MS/MS)
Findings release: on the same working day, via LIMS
DrugBank: https://go.drugbank.com/drugs/DB00321